The impact of four harvesting techniques on the cell viability and osteogenic behaviour of cells in autogenous bone grafts : a critical appraisal of an experimental study

摘要

The investigators tested the null hypothesis that there would be no differences between thedifferent bone harvesting techniques with regard to cell viability, cell activity and osteogenicpotential of grafted cells. Bone grafts were harvested from the mandibles of 12 miniature pigsusing four different harvesting techniques: bone milling, bone scraping, bone drilling (boneslurry) and piezosurgery. Cell viability was determined according to an immunoassay ofreleased signalling molecules and gene expression that affect bone formation and resorption.The osteogenic activity of conditioned graft-sampled media was assessed in a bioassay usingisolated bone cells. Cells in autogenous bone grafts obtained by using a bone mill and abone scraper showed a higher viability and a stronger osteogenic potential than those frompiezosurgery and bone drilling (slurry). This study contributed towards the understanding ofthe impact of harvesting techniques on the viability and osteogenic behaviour of grafted cells.